Table A2.
Characteristics of six microsatellite loci from samples of Siganus spinus recruits and adults (>100 mm FL) from Guam. Given are: Collection code and sample size (N), number of alleles (A), observed heterozygosity (HO), expected heterozygosity (HE), and inbreeding coefficient (FIS)1
Sample code (N) | Sfus-113 | Sfus-56 | Sfus-8 | Sfus-9 | Sfus-98 | Sfus-21 | |
---|---|---|---|---|---|---|---|
AchN7 | A | 10 | 1 | 11 | 1 | 3 | 6 |
20 | HO | 0.737 | 0.000 | 0.471 | 0.000 | 0.100 | 0.750 |
HE | 0.706 | 0.000 | 0.841 | 0.000 | 0.096 | 0.713 | |
FIS | 0.070 | NA | 0.554 | NA | –0.013 | –0.027 | |
AgaN7 | A | 6 | 2 | 12 | 2 | 2 | 5 |
13 | HO | 0.846 | 0.077 | 0.538 | 0.077 | 0.154 | 0.692 |
HE | 0.722 | 0.074 | 0.882 | 0.074 | 0.142 | 0.707 | |
FIS | –0.133 | 0.000 | 0.423 | 0.000 | –0.044 | 0.061 | |
AgaS8 | A | 9 | 2 | 13 | 1 | 2 | 7 |
20 | HO | 0.900 | 0.050 | 0.700 | 0.000 | 0.100 | 0.700 |
HE | 0.806 | 0.049 | 0.858 | 0.000 | 0.095 | 0.748 | |
FIS | –0.091 | 0.000 | 0.208 | NA | –0.027 | 0.089 | |
CocA | A | 9 | 2 | 12 | 2 | 3 | 7 |
24 | HO | 0.875 | 0.042 | 0.565 | 0.042 | 0.333 | 0.542 |
HE | 0.803 | 0.041 | 0.857 | 0.041 | 0.288 | 0.616 | |
FIS | –0.069 | 0.000 | 0.394 | 0.000 | –0.136 | 0.142 | |
EAgN7 | A | 8 | 2 | 8 | 1 | 2 | 6 |
16 | HO | 1.000 | 0.125 | 0.429 | 0.000 | 0.125 | 0.813 |
HE | 0.801 | 0.117 | 0.829 | 0.000 | 0.117 | 0.693 | |
FIS | –0.218 | –0.035 | 0.582 | NA | –0.035 | –0.140 | |
EAgS8 | A | 10 | 1 | 13 | 2 | 3 | 6 |
21 | HO | 0.762 | 0.000 | 0.429 | 0.048 | 0.238 | 0.762 |
HE | 0.787 | 0.000 | 0.887 | 0.046 | 0.214 | 0.662 | |
FIS | 0.056 | NA | 0.534 | 0.000 | –0.087 | –0.127 | |
GovA | A | 8 | 2 | 13 | 1 | 3 | 7 |
26 | HO | 0.769 | 0.038 | 0.542 | 0.000 | 0.423 | 0.577 |
HE | 0.737 | 0.038 | 0.891 | 0.000 | 0.341 | 0.616 | |
FIS | –0.024 | 0.000 | 0.461 | NA | –0.222 | 0.083 | |
GovO8 | A | 8 | 2 | 10 | 2 | 2 | 7 |
20 | HO | 0.737 | 0.050 | 0.444 | 0.050 | 0.100 | 0.550 |
HE | 0.745 | 0.049 | 0.847 | 0.049 | 0.095 | 0.626 | |
FIS | 0.113 | 0.000 | 0.556 | 0.000 | –0.027 | 0.147 | |
GovS8 | A | 9 | 2 | 11 | 1 | 2 | 6 |
26 | HO | 0.808 | 0.038 | 0.478 | 0.000 | 0.115 | 0.538 |
HE | 0.752 | 0.038 | 0.882 | 0.000 | 0.174 | 0.531 | |
FIS | –0.054 | 0.000 | 0.541 | NA | 0.353 | 0.006 | |
IpaA | A | 11 | 2 | 14 | 2 | 3 | 6 |
42 | HO | 0.805 | 0.024 | 0.615 | 0.024 | 0.167 | 0.732 |
HE | 0.761 | 0.024 | 0.889 | 0.024 | 0.194 | 0.682 | |
FIS | –0.006 | 0.000 | 0.375 | 0.000 | 0.151 | –0.013 | |
IpaN7 | A | 8 | 2 | 10 | 2 | 2 | 5 |
13 | HO | 0.727 | 0.077 | 0.500 | 0.077 | 0.077 | 0.462 |
HE | 0.802 | 0.074 | 0.833 | 0.074 | 0.074 | 0.444 | |
FIS | 0.299 | 0.000 | 0.489 | 0.000 | 0.000 | 0.000 | |
IpaS8 | A | 10 | 1 | 10 | 1 | 2 | 6 |
19 | HO | 0.789 | 0.000 | 0.368 | 0.000 | 0.316 | 0.579 |
HE | 0.755 | 0.000 | 0.853 | 0.000 | 0.266 | 0.683 | |
FIS | –0.019 | NA | 0.586 | NA | –0.161 | 0.178 | |
PagA | A | 9 | 2 | 6 | 1 | 2 | 5 |
11 | HO | 0.818 | 0.091 | 0.500 | 0.000 | 0.364 | 0.545 |
HE | 0.789 | 0.087 | 0.765 | 0.000 | 0.298 | 0.636 | |
FIS | 0.011 | 0.000 | 0.468 | NA | –0.177 | 0.189 | |
PagS8 | A | 9 | 1 | 13 | 1 | 3 | 5 |
22 | HO | 0.864 | 0.000 | 0.667 | 0.000 | 0.136 | 0.636 |
HE | 0.774 | 0.000 | 0.872 | 0.000 | 0.129 | 0.661 | |
FIS | –0.093 | NA | 0.299 | NA | –0.033 | 0.061 | |
RioJ8 | A | 8 | 1 | 10 | 1 | 2 | 7 |
15 | HO | 0.733 | 0.000 | 0.333 | 0.000 | 0.200 | 0.600 |
HE | 0.773 | 0.000 | 0.880 | 0.000 | 0.180 | 0.562 | |
FIS | 0.086 | NA | 0.642 | NA | –0.077 | –0.033 | |
RioO8 | A | 5 | 1 | 8 | 1 | 1 | 5 |
8 | HO | 0.800 | 0.000 | 0.375 | 0.000 | 0.000 | 0.500 |
HE | 0.740 | 0.000 | 0.836 | 0.000 | 0.000 | 0.609 | |
FIS | 0.398 | NA | 0.596 | NA | NA | 0.243 | |
RioS8 | A | 12 | 1 | 14 | 1 | 3 | 6 |
48 | HO | 0.771 | 0.000 | 0.548 | 0.000 | 0.292 | 0.563 |
HE | 0.733 | 0.000 | 0.894 | 0.000 | 0.259 | 0.641 | |
FIS | –0.041 | NA | 0.478 | NA | 0.463 | 0.134 | |
TanA | A | 10 | 2 | 13 | 1 | 3 | 7 |
33 | HO | 0.719 | 0.030 | 0.594 | 0.000 | 0.152 | 0.697 |
HE | 0.793 | 0.030 | 0.900 | 0.000 | 0.142 | 0.685 | |
FIS | 0.149 | 0.000 | 0.377 | NA | –0.053 | –0.003 | |
TanJ8 | A | 8 | 1 | 8 | 1 | 3 | 7 |
19 | HO | 0.632 | 0.000 | 0.474 | 0.000 | 0.263 | 0.789 |
HE | 0.673 | 0.000 | 0.845 | 0.000 | 0.234 | 0.716 | |
FIS | 0.089 | NA | 0.461 | NA | –0.098 | –0.076 | |
TanN7 | A | 8 | 1 | 7 | 1 | 2 | 4 |
9 | HO | 0.778 | 0.000 | 0.556 | 0.000 | 0.222 | 0.667 |
HE | 0.784 | 0.000 | 0.796 | 0.000 | 0.198 | 0.574 | |
FIS | 0.067 | NA | 0.355 | NA | –0.067 | –0.103 | |
TanO8 | A | 8 | 1 | 11 | 1 | 2 | 6 |
23 | HO | 0.826 | 0.000 | 0.545 | 0.000 | 0.000 | 0.652 |
HE | 0.787 | 0.000 | 0.870 | 0.000 | 0.083 | 0.680 | |
FIS | –0.027 | NA | 0.425 | NA | 1.000 | 0.063 | |
TangS8 | A | 8 | 1 | 11 | 2 | 2 | 7 |
19 | HO | 0.789 | 0.000 | 0.526 | 0.053 | 0.105 | 0.737 |
HE | 0.770 | 0.000 | 0.875 | 0.051 | 0.188 | 0.652 | |
FIS | 0.002 | NA | 0.421 | 0.000 | –0.116 | –0.103 |
Numbers in bold typeface indicate significant departure from Hardy–Weinberg equilibrium at the 0.05 level after Bonferroni correction.