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Journal of Virology logoLink to Journal of Virology
. 1978 Jan;25(1):78–85. doi: 10.1128/jvi.25.1.78-85.1978

In Vitro Translation of the Two RNAs of Nodamura Virus, a Novel Mammalian Virus with a Divided Genome

J F E Newman 1,, T Matthews 1,††, D R Omilianowski 1, T Salerno 1, P Kaesberg 1, R Rueckert 1
PMCID: PMC353903  PMID: 621788

Abstract

Nodamura virus is a small ribovirus containing two RNA molecules. Both RNAs were found to be active messengers for protein synthesis in cell-free extracts prepared from wheat embryo or HeLa cells. RNA 2 directed synthesis of a 43,000-dalton product, p43, whose tryptic fingerprint was similar to that of the major viral coat protein, vp40 (molecular weight, 40,000). Though p43 appears to be a precursor of vp40, processing did not occur in the cell-free extracts. RNA 1 directed synthesis of a 105,000-dalton protein, p105. Its tryptic fingerprint revealed no evidence of coat protein sequences. Hence, the two RNAs represent genes with few, if any, redundant coding sequences.

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Selected References

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