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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2012 Nov 6;54:62–70. doi: 10.1016/j.freeradbiomed.2012.10.556

Fig. 6.

Fig. 6

Binding of Prdx6 with recombinant purified GSTP1-1 allelic variants in vitro. Panel A: titration of 2.0nM Alexa®546-labeled Prdx6 with QSY®35-labelled and GSH-loaded GSTP1-1 allelic variants; effects of labeled Prdx6 pre-incubation with 250nM unlabeled GSTP1-1A; and omission of GSTP1-1 pre-incubation with GSH. Panel B: Hyperbolic fit of experimental data (see Materials and Methods) for initial titrations of GSTP1 allelic variants. Panel C: statistical differences in affinity (KD) of GSTP1 allelic variants for Prdx6. Data represent mean ±SE for 5 independent experiments, &-represents p≤ 0.001. Panel D: Volume of polymorphic amino acids for GSTP1-1allelic variants. Total volume (cubic angstroms) is calculated as a function of variable (105 and 114) amino acid specific volumes comparison with GSTP1-1A variant.