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. 2013 Jan 8;3:1040. doi: 10.1038/srep01040

Figure 2. Voltage-dependent response and pharmacology of injected samples produced from cell-free translation.

Figure 2

(a) SDS-PAGE analysis of proteins produced by wheat-germ cell-free translation. Lanes 1 to 4, representative gel slices from different experiments displaying, from left to right, the molecular weight marker (in kDa), GFP-cytb5t (32 kDa), wildtype and M356C zh4IR channel monomers (71 kDa). The protein samples shown in the gel correspond to those used in other experiments shown in this figure. The family of current traces elicited in response to depolarizing voltage steps (−80 to +60 mV, Δ20 mV) for uninjected oocytes and oocytes injected with GFP-cytb5t (at 0.86 pmol) (b) were negligible compared to the wildtype channel (c) and the M356C channel (d) injected at 0.19 pmol. Current-voltage (I–V) relationship for wildtype (e) and mutant (f) Shaker channels. Inhibition of wildtype (g) and mutant (h) channels in the absence (black) and presence (red) of 10 nM of the pore blocking compound AgTx-2.