FIG. 4.
Expression levels of phospho-MADD (pMADD) and phospho-Akt (pAkt) upon TRAIL treatment in thyroid cancer cell lines: total of 6×105 of (A) BCPAP, (B) TPC1, (C) KTC1, and (D) FTC133 thyroid cancer cells were cultured in 60-mm dishes overnight and treated with TRAIL at the concentration and for the duration indicated. Cell lysates containing 50 μg of protein were subjected to SDS-PAGE and transferred to nitrocellulose membranes. Blots were stained using specific antibodies against Akt, pAkt, MADD, and pMADD. The protein bands were visualized by enhanced chemiluminescence.