FIG. 2.

Total interferon alpha expression patterns are different in (A) brain (parietal cortex), (B) lung, and (C) spleen. RT-PCR was done with interferon alpha primers that spanned conserved regions among all alpha subtypes on uninfected animals, as well as animals sacrificed at 7, 10, 14, and 21 days postinfection, with at least 5 animals per group. All values are normalized to 18 s ribosomal RNA and expressed as a fold induction over uninfected controls. Solids lines represent medians. All reactions were done with duplicate technical replicates in 3 independent experiments. One representative experiment is shown.