FIG. 3.

Interferon alpha subtype expression in brain. RT-PCR assays for all interferon alpha subtypes were run in the parietal cortex in uninfected, 7 and 42 days animals, with alphas (A) 13, (B) 2, and (C) 6 being expressed. At least 4 animals were run per group. All values are normalized to 18 s ribosomal RNA and expressed as a fold induction over uninfected controls. Solid lines represent medians. All samples were run with duplicate technical replicates in 3 independent experiments. One representative experiment is shown. (D) Ct values for all uninfected (solid gray bars) and 7-day (shaded bars) animals are graphed for alphas 6, 13, and 2.