Table 2.
Real-Time Polymerase Chain Reaction Primer and Probe Sequences Based on Pigtailed Macaque Alpha Sequences
| Alpha subtype | F (5′-3′) | R (5′-3′) | Probe |
|---|---|---|---|
| 1/13 | CCATCTCTGTCCTCCATGTGC (56) | GCTTCCAAGTCATTCAGCTGCT (55) | AGACTCATCTGCTGCTTGGGATGAGGAC (63) |
| 2 | CTGAAGGACAGACATGACTTGGA (55) | TCTCATGGAGGACAGGGATTGT (55) | CCCAGGAGGAGTTTGGCAACCAGT (63.3) |
| 6 | ATGAGGTGATCCAGCAGACGTA (55) | TTCAGTGTAGAGTTTGTCTAGAACCA (55) | CAGCACAAAGGACTCATCTGCTGCTTGG (63) |
| 8 | CCTGAAGGACAGACATGACTTGA (55) | CTGGTCAAGTTCGATGTAGAATGCA (56) | CCCCAGGAGGAGTTTGATGACAAAAACTTCC (62) |
| 21 | TCCCCCAGGAGGAGTTTGATG (56) | ATTCAGTTGCTGGTAAAGTCCCGT (56) | CTGCTGCTTGGGAACACAGCCTCCTAGAA (65) |
| 17 | CTCTGGGCTGTGATCCTACC (56) | CAAAGTCATGTCTGTCCTTCCGA (55) | CTGGGTCATAGGAGGGCCTTGATACTCC (63) |
| 14 | CCTGAAGGACAGAAATGACTTCGA (56) | TTCCAAGTCATTCAGTTGCTCGA (55) | CAGCACAAAGGACTCATCTGCTGCTTGG (63) |
| 4 | CTCTCTGGGCTGTGATCTTACT (54) | CCTGGGGGAATCCAAAGTAAC (55) | CTGGGTCATAGGAGGGCCTTGATACTCC (63) |