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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: J Endocrinol. 2012 Mar 28;213(3):251–261. doi: 10.1530/JOE-11-0406

Figure 1.

Figure 1

Fat accumulation in rat hepatocytes increases with age. (A) Liver sections from ten young (6 months old) and seven old (24 months old) Fischer 344 male rats were stained with ORO and hematoxylin. (Left panel) Representative liver sections from one young and one old rat are shown. (Right panel) Quantitation of fat content in the liver sections, as described in Materials and methods, indicates a 3·7-fold increase in tissues from old rats. Data are presented as mean values ±s.e.m. (*P<0·0001 vs liver fat content in young animals). (B) Liver sections from the same group of young and old animals used to measure fat content in (A) were stained with hematoxylin and eosin and scored as grades 0, 1, 2, or 3 based on fatty change as previously described (Maeda et al. 1985). (Upper panel) Representative liver sections for each grade are shown. (Lower panel) Table depicting the percentage of young and old rat liver tissues graded as 0, 1, 2, and 3. Full colour version of this figure available via http://dx.doi.org/10·1530/JOE-11-0406.