Principal component analysis of the gene expression profile. The transcriptional profiles are shown of the neonatal brain of mice conceived by ICSI, IVF or natural mating (NM) and embryo transfer to the surrogate mothers. Vertical axis (PCA1) and horizontal axis (PCA2) are principal component 1 and 2, respectively, of the gene expression profiles obtained by DNA microarray. Genetic background of all neonates are B6×D2. Strain name of surrogate mothers (B6 or ICR) are indicated in parentheses. NM: natural mating and continued the pregnancy. NM (B6): natural mating, flush out embryos and transplant to B6 surrogate mothers. NM (ICR); natural mating, flush out embryos and transplant to ICR surrogate mothers.