Figure 2.

Molecular barcoded yeast collections for characterizing the mechanism of bioactive chemical compounds. A pool of barcoded strains is grown in the presence (bottom) or absence (top) of a chemical inhibitor. Each strain in the pool contains a unique barcode (represented by different colors). Cells are collected, DNA extracted, and barcodes are then PCR amplified using common primers (common primer sites are shown in grey). The individual barcodes in each sample are then quantified, using a tag array or sequencing, and results +/− chemical inhibitor are compared.