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. 2011 Apr 1;17(4):CR227–CR234. doi: 10.12659/MSM.881720

Figure 5.

Figure 5

Up-regulation of adhesion molecule mRNA expression in human umbilical vein endothelial cells (HUVECs) by interleukin-17A (IL-17A) derived from patients with active Behçet’s disease (BD). (A): PBMCs from patients with active BD (n=20), MS (n=12), SLE (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay. (B): Quantitative reverse transcription-polymerase chain reaction was performed to determine the expression of adhesion molecule mRNA in HUVECs induced by supernatants from active BD patients from the culture of peripheral blood mononuclear cells (PBMCs) derived from patients with active BD (n=10).