Figure 3.

Viability of human gingival fibroblasts exposed for 6 h at 37°C to the mixture of methacrylate monomers containing 55% bisphenol A-diglycidyl dimethacrylate and 45% 2-hydroxyethyl methacrylate (w/w) (Bis-GMA/HEMA) at 0.1 mM Bis-GMA with or without a 1 h preincubation with chitosan oligosaccharide lactate (ChOL) at indicated concentrations. The viability was measured by flow cytometry with thiazole orange and propidium iodide. Displayed is the mean of three experiments of 5×10⁴ measurements each, error bars denote standard deviation. The inset presents the viability of the cells exposed to ChOL singly. ** p<0.01, * p<0.05 as compared with control without preincubation with ChOL.