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. 2013 Jan 7;3:180. doi: 10.3389/fendo.2012.00180

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Copyright © Chen, Van Hover, Lindberg and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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(A) Darkfield photomicrograph showing the injection site (green circle) of an adenoviral vector encoding Cre-regulated expression of farnesylated green fluorescent protein (GFP_f) delivered into the VMH of a CRFR2-Cre mouse. The expression of GFP_f is normally silenced due to a Cre-regulated transcription block sequence inserted upstream of GFP_f reporter cassette. In the presence of Cre, the transcription block sequence is removed to allow GFP_f to be expressed in Cre-positive cells. (B) Darkfield photomicrograph depicting the distribution of VMH CRFR2-positive fibers (golden staining) within the rostral ventrolateral medulla (RVLM). ARH, arcuate nucleus of hypothalamus; MARN, magnocellular reticular nucleus; ME, median eminence; Py, pyramidal tract; Tu, tuberal nucleus; V3, third ventricle; VII, facial nucleus; VMHdn, dorsomedial part of the ventromedial nucleus of hypothalamus. Scale bar = 50 μm.