FIGURE 7.
Upregulation of A2BAR in MS patients and the effect of CVT-6883 on human DCs. (A) The expression of ARs in PBLs from normal controls (n = 20) and patients with RRMS (n = 10) was analyzed with qPCR. (B) Human DCs from healthy donors were stimulated with anti-CD4 (10 μg/ml) for 36 h alone, in combination with NECA (10 μM), or in combination with NECA and CVT-6883 (10 μM), and IL-6 production was measured. (C) Human DCs were incubated with PLCβ inhibitor U73122 (10 μM) or PKC inhibitor Ro 31-8220 (1 μM) for 30 min, then stimulated with anti-CD40 and NECA (10 μM) for 36 h, and IL-6 production was measured. Data are mean ± SEM (n = 3). **p < 0.01 versus naive control, ***p < 0.001 versus anti-CD40 alone, ###p < 0.001 versus NECA treatment.