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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: Nature. 2011 Mar 30;473(7347):389–393. doi: 10.1038/nature09934

Figure 4. Tet1 is required for chromatin binding of PRC2 in mouse ES cells.

Figure 4

a, Tet1 depletion affects the binding of PRC2 to the majority of its targets. PRC2-binding sites are divided into three groups (Tet1/PRC2 co-bound Tet1 dependent, Tet1 independent and PRC2-only bound). b, Shown are Tet1, Ezh2 and Suz12 (ref. 21), and H3K27me3 (ref. 13) occupancy, and the effect of Tet1 depletion on Ezh2 occupancy and 5mC levels at seven representative Tet1-repressed bivalent targets. Regions associated with significant changes in Ezh2 occupancy between control and Tet1-depleted ES cells were measured by whole-genome tiling microarrays. Genomic regions that are further examined by locus-specific ChIP–qPCR in c are shaded. c, ChIP–qPCR analysis of Tet1 (top panels) and Ezh2 (bottom panels) occupancy at the promoters of eight representative Tet1-repressed targets in control (Con KD), Tet1-depleted (Tet1 KD) and Ezh2-depleted (Ezh2 KD) ES cells. Error bars represents standard deviation determined from duplicate experiments.