Tab 1.

Oligonucleotide primers used for cDNA amplification.

mRNA	Sense primer	Antisense primer	Anneal temperature (°C)	PCR target (bp)	Cycles
TGF-β1	5′-CTG TCC AAA CTA AGG CTC GC-3′	5′-AG A CAG CCA CTC AGG CGT A-3′	55	432	38
Col IV	5′-ATT CCT TTG TGA TGC ACA CCA G-3′	5′-AAG CTG TAA GCA TTC GCG TAG TA-3′	56	151	38
TRPC6	5′-AAA GAT ATC TTC AAA TTC ATG GTC-3′	5′-CAC GTC CGC ATC ATC CTC AAT TTC-3′	54	327	38
β-actin	5′-AGC ATT TGC GGT GCA CGA TGG AGG G-3′	5′-ATG CCA TCC TGC GTC TGG ACC TGG C-3′	53	606	38