Table 1.
Expression of several groups of genes directly related to the BBB function
| Probe set identification | Gene symbol | Gene name | Neonatal |
Adult |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Contra | Ipsi | Ipsi/contra | Contra | Ipsi | Ipsi/contra | |||||
| Pan endothelial genes | ||||||||||
| 1393067_at | Tek | Endothelial-specific receptor tyrosine kinase (Tie2) | 11,647 | 11,985 | 1.0 | 12,776 | 14,525 | 1.1 | ||
| Tight junctions | ||||||||||
| 1374104_at | Cldn5 | Claudin 5 | 24,730 | 23,434 | 0.9 | 20,181 | 17,964 | 0.9 | ||
| 1368115_at | Cldn3 | Claudin 3 | 95 | 100 | 1.0 | 153 | 128 | 0.8 | ||
| 1373182_at | Cldn12 | Claudin 12 | 418 | 308 | 0.7 | 531 | 485 | 0.9 | ||
| 1378753_at | Ocln | Occludin | 9600 | 7132 | 0.7 | 8047 | 3034 | 0.4 | a | |
| 1372473_at | Tjp1 | Tight junction protein 1 (ZO-1) | 13,473 | 12,329 | 0.9 | 12,887 | 10,971 | 0.9 | ||
| 1370940_at | Tjp2 | Tight junction protein 1 (ZO-2) | 3237 | 3503 | 1.1 | 2664 | 4112 | 1.5 | ||
| 1384143_at | Cgn | Cingulin | 334 | 62 | 0.2 | a | 135 | 117 | 0.9 | |
| 1389107_at | Cgnl1 | Cingulin-like 1 | 11,857 | 8856 | 0.7 | 9625 | 9345 | 1.0 | ||
| Adhesion molecules | ||||||||||
| 1391946_at | Selp | P-selectin | 39 | 1094 | 28.1 | a | 43 | 400 | 9.4 | |
| 1388018_at | Sele | E-selectin | 22 | 1255 | 57.6 | b | 21 | 4414 | 214.3 | b |
| 1387202_at | Icam1 | Intracellular adhesion molecule 1 | 1471 | 5637 | 3.8 | 646 | 1892 | 2.9 | ||
| 1368474_at | Vcam1 | Vascular cell adhesion molecule 1 | 593 | 2058 | 3.5 | 747 | 807 | 1.1 | ||
| 1370043_at | Alcam | Activated leukocyte adhesion molecule | 962 | 489 | 0.5 | 890 | 620 | 0.7 | ||
| Extracellular matrix components | ||||||||||
| 1370927_at | Col12a1 | Collagen, type XII, α 1 | 60 | 423 | 7.0 | a | 65 | 112 | 1.7 | |
| 1388939_at | Col15a1 | Collagen, type XV, α 1 | 114 | 707 | 6.2 | a | 90 | 119 | 1.3 | |
| 1388459_at | Col18a1 | Collagen, type XVII, α 1 | 1712 | 5573 | 3.3 | 364 | 925 | 2.5 | ||
| 1387854_at | Col1a2 | Collagen, type I, α 2 | 273 | 295 | 1.1 | 156 | 235 | 1.5 | ||
| 1377631_at | Col9a3 | Collagen, type IX, α 3 | 8493 | 3315 | 0.4 | 707 | 181 | 0.3 | ||
| 1370895_at | Col5a2 | Collagen, type V, α 2 | 7215 | 6924 | 1.0 | 1572 | 1498 | 1.0 | ||
| 1374705_at | Col4a5 | Collagen, type IV, α 5 | 9183 | 7435 | 0.8 | 5664 | 3523 | 0.6 | ||
| 1388494_at | Col4a2 | Collagen, type IV, α 2 | 15,643 | 16,910 | 1.1 | 1478 | 6966 | 4.7 | a | |
| 1372439_at | Col4a1 | Collagen, type IV, α 1 | 15,622 | 19,599 | 1.3 | 3278 | 13,571 | 4.1 | a | |
| 1370959_at | Col3a1 | Collagen, type III, α 1 | 948 | 527 | 0.6 | 70 | 255 | 3.6 | a | |
| 1368380_at | Vtn | Vitronectin | 1324 | 246 | 0.2 | 2701 | 396 | 0.1 | ||
| 1370234_at | Fn1 | Fibronectin 1 | 19,206 | 19,778 | 1.0 | 14,529 | 9728 | 0.7 | ||
| 1390404_at | Lama2 | Laminin, α 2 | 1423 | 292 | 0.2 | 1066 | 495 | 0.5 | ||
| 1392926_at | Lama1 | Laminin, α 1 | 1215 | 611 | 0.5 | 142 | 84 | 0.6 | ||
| 1388932_at | Lama5 | Laminin, α 5 | 1079 | 1413 | 1.3 | 656 | 1618 | 2.5 | a | |
| 1398275_at | Mmp9 | Matrix metallopeptidase 9 | 187 | 88 | 0.5 | 42 | 2631 | 63.2 | a | |
| 1370301_at | Mmp2 | Matrix metallopeptidase 2 | 131 | 165 | 1.3 | 229 | 101 | 0.4 | ||
| Angiogenesis regulators | ||||||||||
| 1368463_at | Vegfc | Vascular endothelial growth factor C | 2221 | 2781 | 1.3 | 1120 | 1004 | 0.9 | ||
| 1380854_at | Vegfb | Vascular endothelial growth factor B | 324 | 249 | 0.8 | 345 | 202 | 0.6 | ||
| 1373807_at | Vegfa | Vascular endothelial growth factor A | 64 | 52 | 0.8 | 156 | 164 | 1.1 | ||
| 1367948_a_at | Kdr | VEGFR-2 | 14,626 | 12,974 | 0.9 | 4151 | 10,916 | 2.6 | a | |
| 1369216_a_at | Flt4 | VEGFR-3 | 95 | 57 | 0.6 | 37 | 40 | 1.1 | ||
| 1370492_a_at | Flt1 | VEGFR-1 | 3655 | 2348 | 0.6 | 1194 | 2417 | 2.0 | ||
| 1374207_at | Angpt2 | Angiopoietin 2 | 9569 | 6066 | 0.6 | 2761 | 14,586 | 5.3 | a | |
| 1381487_at | Angpt1 | Angiopoietin 1 | 14 | 14 | 1.0 | 16 | 13 | 0.8 | ||
| 1398358_a_at | Itgb5 | Integrin β 5 | 250 | 211 | 0.8 | 442 | 204 | 0.5 | ||
| 1368819_at | Itgb1 | Integrin β 1 | 10,566 | 11,530 | 1.1 | 8654 | 11,736 | 1.4 | ||
| 1370937_a_at | Itga7 | Integrin α 7 | 323 | 199 | 0.6 | 377 | 163 | 0.4 | ||
| 1385649_at | Itga5 | Integrin α 5 | 240 | 232 | 1.0 | 48 | 60 | 1.2 | ||
| 1387144_at | Itga1 | Integrin α 1 | 2723 | 1880 | 0.7 | 767 | 637 | 0.8 | ||
| Transporters | ||||||||||
| 1388750_at | Tfrc | Transferrin receptor | 20,171 | 6609 | 0.3 | 7354 | 3660 | 0.5 | ||
| 1370848_at | Slc2a1 | Glut-1 | 15,273 | 16,006 | 1.0 | 21,097 | 19,609 | 0.9 | ||
| 1386981_at | Slc16a1 | MCT1 | 18,730 | 9408 | 0.5 | 4735 | 4156 | 0.9 | ||
| 1387280_a_at | Slc7a5 | TA1 | 3950 | 735 | 0.2 | 678 | 313 | 0.5 | ||
| 1368391_at | Slc7a1 | CAT1 | 17,439 | 8603 | 0.5 | 11,275 | 5545 | 0.5 | ||
| 1370367_at | Slc1a1 | EEAC1 | 94 | 55 | 0.6 | 1475 | 2113 | 1.4 | ||
| 1368461_at | Slc22a8 | OAT3 | 7157 | 1153 | 0.2 | 1276 | 166 | 0.1 | ||
| 1387970_at | Slc38a5 | JM24 | 9584 | 8516 | 0.9 | 396 | 648 | 1.6 | ||
| 1370824_at | Slc38a3 | Snat3 | 6706 | 3092 | 0.5 | 1622 | 693 | 0.4 | ||
| 1368295_at | Slco2b1 | OATP-B | 2418 | 1320 | 0.5 | 1831 | 1017 | 0.6 | ||
| 1370205_at | Slco1c1 | OATP-F | 24,648 | 12,147 | 0.5 | 10,052 | 823 | 0.1 | ||
| 1387093_at | Slco1a4 | Oatp2 | 4272 | 1859 | 0.4 | 23,989 | 7497 | 0.3 | ||
| 1380577_at | Abcg2 | Bcrp | 10,466 | 5774 | 0.6 | 13,279 | 2549 | 0.2 | ||
| 1370583_s_at | Abcb1a, Abcb1b | P-glycoprotein | 2472 | 1078 | 0.4 | 1362 | 604 | 0.4 | ||
| 1370465_at | Abcb1a | P-glycoprotein | 4269 | 1965 | 0.5 | 4974 | 1941 | 0.4 | ||
| Wnt mediators | ||||||||||
| 1387184_at | Axin2 | Axin 2 | 312 | 69 | 0.2 | 70 | 42 | 0.6 | ||
| 1383785_at | Lef1 | Lymphoid enhancer binding factor 1 | 3283 | 3689 | 1.1 | 3071 | 2338 | 0.8 | ||
| 1388994_at | Fzd6 | Frizzled 6 | 5802 | 6912 | 1.2 | 1488 | 1836 | 1.2 | ||
| 1373067_at | Ctnnb1 | b-catenin | 4409 | 5293 | 1.2 | 8146 | 6466 | 0.8 | ||
aStatistical significance: only this age.
bStatistical significance: both ages.