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Swapping of mini domains within Domain III between MHV and other CoV nsp15s. (A) Diagram showing the replacement of the mini domain in Domain III of SARS-CoV and TGEV nsp15 with the corresponding mini domain of MHV nsp15, and the resultant chimeric constructs. Only the nsp15 region of the EGFP fusion construct is shown. (B) Expression and detection of speckle for the chimeric constructs.
