Fig. 10.

Effect of ectopically expressed EGFP-nsp15s on MHV replication. DBT cells were transfected with 100 ng each plasmid DNA containing either the full-length or deletion mutant (DI and DIII) nsp15s fused to EGFP as indicated at the bottom of the x-axis. Cells transfected with the vector plasmid expressing EGFP alone were used as a negative control. At 9 h post transfection, the cells were infected with MHV-2aFLS at m.o.i. of 5. Luciferase activity was determined at 14 h p.i., and was expressed as percent relative to the negative control, which is set as 100%. The results were analyzed for statistical significance between the testing and control groups using the Student's t-test. P values of <0.01 or <0.05 were considered statistically significant, which is indicated with an asterisk.