Table 2.

Evaluation of PCR inhibition via bacterial 16S rRNA gene amplification in undiluted (1x) versus diluted DNA extracts^a

	1xb	1:10xb	1:100xc
Sample A
P	2.03±0.15x109	2.07±0.02x109	1.32±0.07x109
Q	6.26±0.57x109	6.06±0.13x109	2.88±0.04x109
M	2.55±0.26x109	2.38±0.10x109	2.46±0.08x109
Sample B
P	8.44±0.17x108	7.33±0.10x108	5.79±0.10x108
Q	6.34±0.56x109	6.32±0.13x109	3.72±0.19x109
M	4.70±0.08x109	5.16±0.20x109	3.95±0.23x109
Sample C
P	6.28±0.43x106	6.83±0.85x106	7.48±0.52x106
Q	9.26±5.48x106	9.59±0.28x106	8.83±0.18x106
M	1.74±0.04x108	1.14±0.05x108	1.15±0.01x108

^aData shown are mean values (n = 3) and standard deviations of 16S rRNA gene copies per ml original sample.

^bStandard curve: m = −3.67; y-int = 41.61; R² = 0.99; Eff = 87.1%.

^cStandard curve: m = −3.59; y-int = 41.15; R² = 0.99; Eff = 89.7%.