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. 2012 Dec 8;11:155. doi: 10.1186/1475-2859-11-155

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Copyright ©2012 Koopman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stepwise increase in naringenin formation byS cerevisiae. Formation of A) naringenin and B) phloretic acid in the engineered strains IMX106 (○)(aro3Δ ARO4^G226Saro10Δ, pdc5Δ, pdc6Δ, atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,at4CL3↑), IMX197 (●)(aro3Δ ARO4^G226Saro10Δ, pdc5Δ, pdc6Δ, atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑, at4CL3↑, cotal1↑) and IMX198 (□) (aro3Δ ARO4^G226Saro10Δ, pdc5Δ, pdc6Δ, atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,coCHS3↑, at4CL3↑, cotal1↑). Cultures were grown in shake flasks on synthetic medium containing 20 g·l^-1 glucose. All cultures were performed in duplicate. Error bar denotes deviation of the mean.