Table 2.
Strains and plasmids used in this study
| Strain | Genotype | Source |
|---|---|---|
| CEN.PK2-1c |
MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2 |
[69] |
| CEN.PK717.5A |
MATa aro3Δ ARO4G226S |
This study |
| IMU011 |
MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2, pUDE172, PYK2::pUDI065 |
This study |
| IMK328 |
MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2 Δaro3(46,1065)::loxP ARO4G226S |
This study |
| IMK389 |
IMK328 pdc6Δ(-6,-2)::loxP pdc5Δ(-6,-2)::loxP |
This study |
| IMK393 |
IMK389 aro10Δ(-6,-2)::loxP |
This study |
| IMX183 |
IMK328 pUDE172, PYK2::pUDI065 |
This study |
| IMX185 |
IMK389 pUDE172, PYK2::pUDI065 |
This study |
| IMX106 |
IMK393 pUDE172, PYK2::pUDI065 |
This study |
| IMX193 |
IMK393 pUDE172, PYK2::pUDI065, TRP1::pUDI069 |
This study |
| IMX197 |
IMK393 pUDE172, PYK2::pUDI065, pUDE188 |
This study |
| IMX198 |
IMK393 pUDE172, PYK2::pUDI065, TRP1::pUDI069, pUDE188 |
This study |
|
Plasmids |
|
|
| pUG6 |
PCR template for loxp-KanMX4-loxp cassette |
[70] |
| pSH47 |
Centromeric plasmid, URA3, PGAL1-Cre-TCYC1 |
[70] |
| pAG416GPD-ccdB |
Centromeric plasmid, URA3, PTDH3-ccdb-TCYC1, Addgene plasmid 14148 |
[71] |
| pAG306GPD-ccdB |
Integration plasmid, URA3, PTDH3-ccdb-TCYC1, Addgene plasmid 14140 |
[71] |
| pAG305GPD-ccdB |
Integration plasmid, LEU2, PTDH3-ccdb-TCYC1, Addgene plasmid 14138 |
[71] |
| pAG304GPD-ccdB |
Integration plasmid, TRP1, PTDH3-ccdb-TCYC1, Addgene plasmid 14136 |
[71] |
| pAG423GPD-ccdB |
2 μm ori, HIS3, PTDH3-ccdb-TCYC1, Addgene plasmid 14150 |
[71] |
| p426TEF |
2 μm ori, URA3, PTEF –TTEF |
[72] |
| p426TPI |
2 μm ori, URA3 PTPI –TADH |
[73] |
| pUNI.U21762 |
Cloning vector, Ka, atCHS3 cDNA# |
[74] |
| pUNI.U11924 |
Cloning vector, Ka, atCHI1 cDNA |
[74] |
| pDZ.4CL3 |
Cloning vector, Bla, at4CL3 cDNA |
[75] |
| pUD168 |
pMA cloning vector, Bla, A-PTDH3-atPAL1-TCYC1-B* |
This study |
| pUD169 |
pMA cloning vector, Bla, B-PPGI-coCPR1-TPGI-C |
This study |
| pUD170 |
pMA cloning vector, Bla, C-PTPI-coC4H-TADH-D |
This study |
| pUD175 |
pMA cloning vector, Bla, E-PPGK-coCHS3-TPGK-F |
This study |
| pUDE185 |
2 μm ori, HIS3, PTDH3-coCHS3-TCYC1 |
This study |
| pUDE186 |
2 μm ori, URA3, PTEF-coCHS3-TTEF |
This study |
| pUDE188 |
2 μm ori, HIS3, PTDH3-coCHS3-TCYC1, PTEF-coCHS3-TTEF |
This study |
| pUDE103 |
2 μm ori, URA3, PTEF-at4CL3-TTEF |
This study |
| pUDE172 |
Centromeric plasmid, URA3, PTDH3-atPAL1-TCYC1, PTPI-coC4H-TADH, PPGI-coCPR1-TPGI |
This study |
| pUDI053 |
Integration plasmid, LEU2, PTDH3-atCHI1-TCYC1 |
This study |
| pUDI060 |
Integration plasmid, LEU2, PTDH3-atCHI1-TCYC1, PTEF-at4CL3-TTEF |
This study |
| pUDI061 |
Integration plasmid, LEU2, PTDH3-atCHI1-TCYC1, PTPI-atCHS3-TADH, PTEF-at4CL3-TTEF |
This study |
| pUDI065 |
Integration plasmid, LEU2, PTDH3-atCHI1-TCYC1, PTPI-atCHS3-TADH, PTEF-at4CL3-TTEF, PYK2(1-710) |
This study |
| pUDI069 | Integration plasmid, TRP1, PTDH3-coTAL1-TCYC1 | This study |
* co: codon optimized, #; at: A. thaliana.