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. 2012 Nov 23;10:71. doi: 10.1186/1477-5956-10-71

Table 3.

Validation of the serum cytokine array experiment in the initial screening cohort

Cytokine Method PSP/CBS MSA PD Controls p-value
ICAM-1
 Array
 0.98 ± 0.25
 0.47 ± 0.09
 0.62 ± 0.15
 1.00 ± 0.28
 <0.05a
(ICAM1)
 FCM
 1.25 ± 0.85
 0.75 ± 0.29
 0.86 ± 0.43
 1.00 ± 0.73
 nsb
IL-2 Ra
 Array
 2.14 ± 0.47
 1.87 ± 0.10
 1.24 ± 0.06
 1.00 ± 0.14
 <0.05a
(IL2RA)
 ELISA
 1.44 ± 0.76
 1.24 ± 0.61
 1.15 ± 0.55
 1.00 ± 0.29
 nsb
Leptin
 Array
 1.93 ± 0.11
 1.13 ± 0.23
 1.91 ± 0.46
 1.00 ± 0.11
 <0.05a
(LEP)
 FCM
 2.95 ± 4.89
 1.42 ± 1.15
 1.86 ± 1.88
 1.00 ± 0.89
 nsb
MCP-4
 Array
 7.18 ± 0.85
 2.16 ± 1.39
 1.71 ± 0.80
 1.00 ± 0.76
 <0.05a
(CCL13)
 ELISA
 1.15 ± 0.32
 0.91 ± 0.37
 1.02 ± 0.32
 1.00 ± 0.42
 nsb
PDGF-BB
 Array
 1.87 ± 0.50
 1.34 ± 0.18
 1.34 ± 0.22
 1.00 ± 0.44
 <0.05a
(PDGFB)
 FCM
 1.42 ± 0.30
 1.32 ± 0.39
 1.11 ± 0.28
 1.00 ± 0.33
 <0.05b
Prolactin
 Array
 4.17 ± 1.74
 1.03 ± 0.15
 0.99 ± 0.08
 1.00 ± 0.19
 <0.05a
(PRL)
 ELISA
 3.06 ± 4.15
 1.39 ± 1.34
 0.71 ± 1.12
 1.00 ± 0.50
 <0.05b
RANTES
 Array
 1.46 ± 0.29
 1.32 ± 0.11
 1.20 ± 0.25
 1.00 ± 0.20
 <0.05a
(CCL5) ELISA 1.13 ± 0.59 0.83 ±0.49 0.94 ± 0.32 1.00 ± 0.51 nsb

Cytokine microarray data were validated by ELISA (MCP-4, prolactin, RANTES and IL-2RA) or by Flow-Cytomix assays (ICAM-1, Leptin and PDGF-BB) in individual samples. All data are shown as means with standard deviations of the ratio to the mean of the control group. Array = Raybiotech cytokine microarray, FCM = Flow-Cytomix, ELISA = enzyme-linked immunosorbent assay, ns = statistically non significant.

P-value: groups were compared using a SAM analysis and b Kruskal-Wallis test.