Table 1.
Sequences of primers used for qMSP and qRT-PCR assays
| qMSP | ||
|---|---|---|
|
GENE |
Primer sequence1 |
Amplicon size (bp)2 |
| SLC6A11 |
MF: TGTTTAGGGTTGGGAAGAAGTTAC |
128 |
| |
MR: ATCGCAATAAACTAAAAAACCTACG |
|
| |
UF: TGTTTAGGGTTGGGAAGAAGTTAT |
133 |
| |
UR: AATAAATCACAATAAACTAAAAAACCTACA |
|
| TUB |
MF: TGGTTGTTAGTTTGATTGTTGTTAC3 |
96 |
| |
MR: AAAACCTATTAAAATTCCCTATATTCG |
|
| |
UF: GTGGTTGTTAGTTTGATTGTTGTTAT |
97 |
| |
UR: CTAAAACCTATTAAAATTCCCTATATTCA |
|
| ALOX12B |
MF: TTCTCTTACCTACCTTAAACCTTCG |
152 |
| |
MR: TGAGATGGAGTTTCGTGTTTTC |
|
| |
UF: TTCTCTTACCTACCTTAAACCTTCA |
154 |
| |
UR: AGTGAGATGGAGTTTTGTGTTTTT |
|
| WNT10B |
MF: TGGGGTGTATAGGTAAAGGTAAATC |
91 |
| |
MR: GAAAATAAATCAAACGAAAACACG |
|
| |
UF: TGGGGTGTATAGGTAAAGGTAAATT |
93 |
| |
UR: TCAAAAATAAATCAAACAAAAACACA |
|
|
qRT-PCR | ||
|
GENE |
Primer sequence |
Amplicon size (bp) |
| ALOX12B |
F: ACCCGAGGGCAAGATGAT |
74 |
| |
R: GCAGGAAGATGGGGCAAT |
|
| SLC6A11 |
F: AGGGGGTACCCATTGCTG |
65 |
| |
R: CTTGGGGTACGCAATAAAGG |
|
| TUB |
F: TCCGACTGGATTCCCTACAG |
109 |
| |
R: GGCGCTTCTTCTTCTGCTT |
|
| WNT10B |
F: GCGAATCCACAACAACAGG |
107 |
| R: TCCAGCATGTCTTGAACTGG | ||
1MF, methylated forward primer; MR, methylated reverse primer; UF, unmethylated forward primer; UR, unmethylated reverse primer; F, forward primer; R, reverse primer.
2 Size of the amplification product in base pairs.
3 Underlined text represent locked nucleic acid (LNA)-modified nucleotides.