Figure 4.

Vacuole morphology changes in P. pastoris GS115 during methanol-induced high-level production of HBsAg. Time-dependent change in the percentage of cells containing large spherical vacuoles with autophagic bodies (AB, ), cells containing large spherical vacuole without autophagic bodies (○), cells containing irregular vacuoles (▴), cells with small vacuoles (Δ), cells without any visible vacuoles (), and apoptotic cells (□). The bar in the electron micrographs of representative cells corresponds to 500 nm.