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. 2013 Jan 8;11(1):e1001465. doi: 10.1371/journal.pbio.1001465

Figure 3. VAB-8 acts in the CAN neurons to regulate epidermal patterning.

Figure 3

(A–C) Expression of vab-8s and CAN promoters from the akEx923 transgenic array in wild-type L2 stage animals. Scale bar is 50 µm. The CANs are a pair of neurons, CANL and CANR, located on the left and right side of each animal, respectively. (A) DsRed2 channel. (B) YFP channel. (C) Merged images from (A) and (B). (D) CAN-specific expression of VAB-8S restores inhibition to vulval fate signaling in vab-8 mutants. vab-8 mutations displace cell bodies and affect axon outgrowth of a subset of neurons including the CANs (blue). VAB-8S was restored to vab-8 mutants with either the CAN-specific promoter (PCAN) or the control minimal pes-10 promoter (Ppes-10). Vulval fates: number of vulval progenitor cells adopting vulval fates. Wild-type is 3.00. p-Value was calculated using a two-tailed Student's t test. Transgenic arrays were dyEx24 (Ppes-10::vab-8s) or dyEx20 (PCAN::vab-8s). CAN-specific expression of VAB-8S from the dyEx20 transgenic array rescues patterning defects in the posterior and anterior epidermis (E and F). p-Value was calculated using a two-tailed Fisher's exact test.