Skip to main content
. 2013 Jan 8;11(1):e1001465. doi: 10.1371/journal.pbio.1001465

Figure 6. Ror/CAM-1 has negative and positive effects on vulval fate signaling.

Figure 6

(A) Ror/CAM-1 is broadly expressed. L2 stage animal with the cwIs6 transgenic array, which expresses a rescuing Ror/CAM-1 translational fusion to GFP from the cam-1 promoter. Red arrows indicate axons of non-CAN neurons. Scale bar is 10 µm. (B) Ror/CAM-1 inhibits Wnt signaling in epidermal progenitors. Quantification of syIs187 Wnt reporter transgene activity in Pn.px stage ror/cam-1 mutants. Pn.px signals were scored as in Figure 4B. (C) Schematic description of ror/cam-1 mutations and the cam-1 transgenic mutant (ΔIntra) used in this study. Numbers indicate amino acid positions. Fz, Frizzled, Wnt-binding extracellular domain; TM, transmembrane domain. (D) Ror/CAM-1 inhibits P3.p from becoming a vulval progenitor in larvae, and helps restrict the Wnt-dependent P12 fate to only the P12 blast cell in embryos. Pegl-20::egl-20 is an integrated transgenic array that introduces extra functional copies of egl-20/wnt fused to gfp into the genome (see Figures S4 and S7). (E) Ror/CAM-1 is required for foreshortening of the posterior CAN axon to promote a vulval fate in the posterior P8.p progenitor. Model depicting negative and positive roles of Ror/CAM-1 on Wnt signaling in epidermal progenitors. p-Values were calculated using a two-tailed Fisher's exact test versus wild-type animals (B and D) or as otherwise indicated (E).