Table 2. CAN displacement causes epidermal phenotypes that require signaling by specific Wnts and that resemble phenotypes caused by mutations in the Wnt-binding Ror/CAM-1 receptor.

Genotype	Vulval Fatesa	n b	p-Valuec
Wild-type	3.00	47
egl-20(lf)	3.00	58
cwn-2(lf)	3.00	25
lin-44(lf)	3.00	20
Wild-type; vector RNAi	3.00	20
Wild-type; cwn-1 RNAi	3.00	20
Wild-type; mom-2 RNAi	3.00	20
let-23(lf); vab-8(gm138)	1.95	20	0.0001 versus let-23(lf)
let-23(lf); egl-20(lf); vab-8(gm138)	0.68	20	0.001 versus let-23(lf); vab-8(gm138)
lin-44(lf); let-23(lf); vab-8(gm138)	2.15	20	0.62 versus let-23(lf); vab-8(gm138)
let-23(lf); cwn-2(lf); vab-8(gm138)	1.30	25	0.07 versus let-23(lf); vab-8(gm138)
let-23(lf); vab-8(gm138); vector RNAi	1.45	20
let-23(lf); vab-8(gm138); cwn-1 RNAi	0.40	31	0.005 versus let-23(lf); vab-8(gm138); vector RNAi
let-23(lf); vab-8(gm138); mom-2 RNAi	0.63	23	0.04 versus let-23(lf); vab-8(gm138); vector RNAi
let-23(lf); syEx1024[Phs::egl-20]	0.13	20
let-23(lf); syEx1024[Phs::egl-20] heat shockd	1.70	20	<0.00001 versus non-transgenic heat shock
Control non-transgenic siblinge	0.00	20
Control non-transgenic sibling heat shockd,e	0.05	20
cam-1(gm122)	3.04	51
cam-1(sa692)	3.03	74
cam-1(ks52)	3.00	62
lin-3(lf)	0.91	46
cam-1(gm122); lin-3(lf)	1.76	44	0.0004 versus lin-3(lf)
cam-1(sa692); lin-3(lf)	2.08	31	<0.00001 versus lin-3(lf)
cam-1(ks52); lin-3(lf)	1.11	27	0.47 versus lin-3(lf)

^aVulval fates: number of vulval progenitor cells adopting vulval fates. Wild-type is 3.00.

^b n: number of animals assayed.

^c p-Values were calculated using a two-tailed Student's t test.

^dMixed-stage animals were heat-shocked at 33°C for 45 min and scored 16 h later.

^eControl animals were of the same genotype as the transgenic animals, but lacked the extrachromosomal transgenic array.

lf, loss-of-function.