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. 2012 Oct 24;12:243. doi: 10.1186/1471-2180-12-243

Figure 2.

Figure 2

Diagrammatic representation of primers location on pvrbp-2 gene. Gray and black boxes indicate intron and exon respectively, and arrows indicate location of primers. F and R: forward and reverse primers of primary PCR respectively, NF and NR: forward and reverse primers of nested PCR respectively. IR1 and IR2 are internal sequencing primers.