Table 2.
Applied antibodies and conditions for primary immunohistochemical reaction
| N-Cadherin | E-Cadherin | P-Cadherin | KSP-Cadherin | |
|---|---|---|---|---|
| Clone |
6 G11 (mouse) |
NCH-38 (mouse) |
56C1 (mouse) |
MRQ-33 (mouse) |
| Source |
Dako, Hamburg |
Dako, Hamburg |
Linaris, Dossenheim |
Zytomed Systems, Berlin |
| |
Germany |
Germany |
Germany |
Germany |
| Pretreatment |
citrate buffer |
citrate buffer |
citrate buffer |
citrate buffer |
| |
pH6.0 40 min |
pH6.0 40 min |
pH6.0 40 min |
pH6.0 40 min |
| Dilution and |
1:50 30 min RT |
1:50 30 min RT |
1:100 30 min RT |
1:50 30 min RT |
| Incubation |
RT: room temperature.