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. 2013 Jan 8;8(1):e53568. doi: 10.1371/journal.pone.0053568

Figure 4. Construction of protein fragments containing HA-13–263 with or without Fd or Fc and analysis of protein expression.

Figure 4

(A) Proteins containing residues of HA-13–263 with Fc (HA-13–263-Fc), with Fd (HA-13–263-Fd-His), or without fusion with Fd and Fc (HA-13–263-His) were constructed. Purified proteins were either boiled at 95°C for 5 min, or not boiled, followed by detection of the expression by SDS-PAGE and Coomassie Blue staining (B), and Western blot (C) by using a HA1-specific mAb. The protein molecular weight marker (kDa) (Invitrogen) is indicated on the left.