Figure 1.
PAR1 induced glutamate release is blocked by CAAC blockers. A) The experimental scheme of in vitro glutamate release assay using isotope-labeled glutamate (3H-glutamate). 30 μM of TFLLR was treated for 6 min to induce glutamate release from cultured astrocytes. B) Bar graphs represent the averaged relative amount of released glutamate by TFLLR treatment (n=12). 100 μM of Niflumic acid (Nif, n=11) or Flufenamic acid (Flu, n=4) was pretreated to block anion channel activation. **p < 0.05 vs. TFLLR-treated group.