Table 1.
Bacteria, Phage and Plasmids
| Strain | Genotype or description | Source |
|---|---|---|
|
Bacteria | ||
| BW23474 |
F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510 |
E. coli Genetic Stock Center (CGSC) # 7838 [39] |
| DH5α |
F-, Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnV44(AS), λ-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17 |
CGSC # 12384 |
| DH5α λpir |
F-, Δ(argF-lac)169,endA1, pir+, recA1 |
Gift from Dr. T. Charles; [40] |
| JM109 |
F', Δ(gpt-lac)0, glnV44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1?, thi-1, hsdR17, pWM5, F128-x |
New England Biolabs |
| W3101 |
F-, λ-, galT22, IN(rrnD-rrnE)1, rph-1 |
CGSC # 4467; |
| W3110 |
F-, λ-, IN(rrnD-rrnE)1, rph-1 |
CGSC # 4474;[41] |
| W3110-Cre (W1NN) |
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-cre (CmR) |
This study |
| W3110-TelN (W2NN) |
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-telN (CmR) |
This study |
| W3110-Tel (W3NN) |
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-tel (CmR) |
This study |
|
Phages | ||
| N15 |
Wild type (wt) (telN+, tos+) |
Gift from Dr. S. Hertwig; [21] |
| P1 |
wt (cre+, loxP+) |
Gift from Dr. B. Funnell; [42] |
| PY54 |
wt (tel+, pal+) |
Gift from Dr. S. Hertwig; [21] |
|
Plasmids | ||
| pAH120 |
attP λ integration plasmid (KnR) |
NBRP [25] |
| pAH123 |
cI857-pL-int Φ80 (ApR) |
NBRP [25] |
| pAH153 |
attP Φ80 integration plasmid (KnR) |
NBRP [25] |
| pBRINT |
lacZ::cat-MCS::lacZ (CmR) |
NBRP; [37] |
| pGL2 |
SV40P-Luc-PolyA-SV40 intron |
Promega |
| pInt(ts) |
cI857-pL-int λ (ApR) |
NBRP [25] |
| pNN1 |
cI857-pR-pL-cre-tL (ApR) |
This study |
| pNN2 |
cI857-pR-pL-tel-tL (ApR) |
This study |
| pNN3 |
cI857-pR-pL-telN-tL (ApR) |
This study |
| pNN4 |
lacZ::cat- cI857-pR-pL-cre-tL::lacZ (CmR) |
This study |
| pNN5 |
lacZ::cat- cI857-pR-pL-tel-tL::lacZ (CmR) |
This study |
| pNN6 |
lacZ::cat- cI857-pR-pL-telN-tL::lacZ (CmR) |
This study |
| pNN7 |
pGL2-egfp switched for luc |
This study |
| pNN8 |
pNN7 + SS (upstream of SV40 promoter) |
This study |
| pNN9 |
pNN8-SS (2SS) (second SS downstream of SV40 polyA sequence) |
This study |
| pNN10 |
pAH120 (SS+) |
This study |
| pNN11 |
pAH153 (SS+) |
This study |
| pPL451 | cI857-pR-pL-MCS-tL (ApR) | Accession # AB248919 National Bioresource Project (NBRP); [43] |