Bacteria
|
BW23474
|
F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510
|
E. coli Genetic Stock Center (CGSC) # 7838 [39]
|
DH5α
|
F-, Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnV44(AS), λ-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17
|
CGSC # 12384
|
DH5α λpir
|
F-, Δ(argF-lac)169,endA1, pir+, recA1
|
Gift from Dr. T. Charles; [40]
|
JM109
|
F', Δ(gpt-lac)0, glnV44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1?, thi-1, hsdR17, pWM5, F128-x
|
New England Biolabs
|
W3101
|
F-, λ-, galT22, IN(rrnD-rrnE)1, rph-1
|
CGSC # 4467;
|
W3110
|
F-, λ-, IN(rrnD-rrnE)1, rph-1
|
CGSC # 4474;[41]
|
W3110-Cre (W1NN)
|
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-cre (CmR)
|
This study
|
W3110-TelN (W2NN)
|
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-telN (CmR)
|
This study
|
W3110-Tel (W3NN)
|
F-, λ-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-tel (CmR)
|
This study
|
Phages
|
N15
|
Wild type (wt) (telN+, tos+)
|
Gift from Dr. S. Hertwig; [21]
|
P1
|
wt (cre+, loxP+)
|
Gift from Dr. B. Funnell; [42]
|
PY54
|
wt (tel+, pal+)
|
Gift from Dr. S. Hertwig; [21]
|
Plasmids
|
pAH120
|
attP λ integration plasmid (KnR)
|
NBRP [25]
|
pAH123
|
cI857-pL-int Φ80 (ApR)
|
NBRP [25]
|
pAH153
|
attP Φ80 integration plasmid (KnR)
|
NBRP [25]
|
pBRINT
|
lacZ::cat-MCS::lacZ (CmR)
|
NBRP; [37]
|
pGL2
|
SV40P-Luc-PolyA-SV40 intron
|
Promega
|
pInt(ts)
|
cI857-pL-int λ (ApR)
|
NBRP [25]
|
pNN1
|
cI857-pR-pL-cre-tL (ApR)
|
This study
|
pNN2
|
cI857-pR-pL-tel-tL (ApR)
|
This study
|
pNN3
|
cI857-pR-pL-telN-tL (ApR)
|
This study
|
pNN4
|
lacZ::cat- cI857-pR-pL-cre-tL::lacZ (CmR)
|
This study
|
pNN5
|
lacZ::cat- cI857-pR-pL-tel-tL::lacZ (CmR)
|
This study
|
pNN6
|
lacZ::cat- cI857-pR-pL-telN-tL::lacZ (CmR)
|
This study
|
pNN7
|
pGL2-egfp switched for luc
|
This study
|
pNN8
|
pNN7 + SS (upstream of SV40 promoter)
|
This study
|
pNN9
|
pNN8-SS (2SS) (second SS downstream of SV40 polyA sequence)
|
This study
|
pNN10
|
pAH120 (SS+)
|
This study
|
pNN11
|
pAH153 (SS+)
|
This study
|
pPL451 |
cI857-pR-pL-MCS-tL (ApR) |
Accession # AB248919 National Bioresource Project (NBRP); [43] |