Figure 3.

PI3K/Akt signaling pathway was involved in the process of adiponectin’s anti-apoptosis in H9c2 cells. Cells were pretreated with 2.5 μg/mL globular adiponectin for 1 h, in the presence or absence of PI3K inhibitor, LY294002, and then exposure to 150 μM palmitate for 12 h. Levels of p-Akt (A and B), t-Akt (A and B), cleaved caspase-3 (C) and PARP (D) were measured by immunoblot and quantitative analysis. These results were reported as mean ± S.E.M. * P< 0.05 versus control, # P< 0.05 versus PA and ※ P< 0.05 versus (PA + gAd).