Figure 5.

Crosstalk between PI3K/Akt and ERK1/2 signaling pathway of palmitate-induced apoptosis in H9c2 cells. Cells were pretreated with LY294002 (10 μM) or U0126 (10 μM) for 1 h, and then treated with 2.5 μg/mL globular adiponectin for 1 h before exposure to 150 μM palmitate for 12 h. Levels of p-ERK1/2 and t-ERK1/2 (A), p-Akt and t-Akt (B) were measured by immunoblot and quantitative analysis. These results were reported as mean ± S.E.M., * P< 0.05 versus control, # P< 0.05 verus PA and ※ P< 0.05 versus (PA + gAd).