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. 2013 Jan 8;8(1):e51842. doi: 10.1371/journal.pone.0051842

Figure 2. TGFβ1 downregulation of Id2 was prevented by combined Smad2/3 knock-down.

Figure 2

HKC 8 cells were transfected with Smad2 and Smad3 siRNAs or negative control siRNA for 24 h. After 24 h serum recovery and 24 h serum free period, the cells were treated with either vehicle (0.1% BSA) or TGFβ1 (5 ng/ml) for a further 24 h. The cells were lysed and the Id2, Smad2 and Smad3 expressions were assessed by immunoblotting. The representative immunoblots show the expression of Id2, Smad2 and Smad3. TGFβ1 downregulation of Id2 was prevented by combined Smad 2/3 knock-down. The data is expressed as mean±SD (n = 6, ns = P>0.05, **<0.01, ***<0.001).