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. 2013 Jan 8;8(1):e51842. doi: 10.1371/journal.pone.0051842

Figure 3. BMP 7 induction of Id2 was prevented by combined Smad1/5 knock-down.

Figure 3

HKC 8 cells were transfected with either Smad1 and Smad5 siRNAs or negative control siRNA for 24 h. After 24 h serum recovery and 24 h serum free period, the cells were treated with either vehicle (0.1% BSA) or BMP 7 (200 ng/ml) for a further 18 h. The cells were lysed and Id2, Smad1 and Smad5 expressions were assessed by immunoblotting. The representative immunoblots show the expression of Id2, Smad1 and Smad5. BMP 7 upregulation of Id2 was prevented by combined Smad1/5 knock-down. The data is expressed as mean±SD (n = 5, ns = P>0.05, ***<0.001).