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. 2013 Jan 8;8(1):e51842. doi: 10.1371/journal.pone.0051842

Figure 4. Id2 down-regulation is the crux of α-SMA induction by TGFβ1.

Figure 4

HKC 8 cells were transfected either with Id2-v5 expression vector or empty vector for 24 h. After 24 h serum free period they were treated either with vehicle (0.1%BSA) or TGFβ1 (5 ng/ml) for 48 h. E-cadherin, α-SMA and Id2 protein expression were assessed by immunoblotting (A). Id2 over expression did not prevent TGFβ1 suppression of E-cadherin (B). TGFβ1 induction of α-SMA was prevented by Id2 overexpression (C). The data is expressed as mean±SD (n = 4, ns = P>0.05,*<0.05, **<0.01, ***<0.001).