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. 2013 Jan 8;8(1):e39441. doi: 10.1371/journal.pone.0039441

Figure 2. Effect of flagellin treatment on hTERT-EECs NF-κB activity.

Figure 2

(A) hTERT-EECs and (B) Ishikawa 3-H-12 cells transfected with the SEAP reporter gene were co-cultured with 0, 10, 100 or 500 ng/ml of flagellin for 24 hours. Samples were collected at 0, 2, 4 and 24 hours and analyzed using Quantiblue™. Data of NF-κB activity are reported as the fold induction of SEAP activity over untreated controls. Different letters mean significant difference (p<0.05). (C) Electrophoretic mobility shift assays (EMSA) analysis of DNA-binding activity of NF-κB in hTERT-EECs treated with (flagellin group) or without (control group) 100 ng/ml of flagellin for 24 hours. Control reactions were incubated with either excess (1000 fold) unlabeled κB (cold specific; CS) or nonspecific cold probe (N) before reaction with labeled NF-κB probe.