Skip to main content
. 2013 Jan 8;8(1):e39441. doi: 10.1371/journal.pone.0039441

Figure 3. Effect of JAr spheres on the endometrial response to flagellin.

Figure 3

A 2×2 factorial experimental design was developed were (A) hTERT-EECs or (B) Ishikawa 3-H-12 cells transfected with the SEAP reporter were either: cultured in the absence of both JAr spheres and flagellin (control); cultured with 100 JAr spheres; cultured with 100 ng/ml of flagellin or pre-incubated with 100 JAr spheres for 1 h before the addition of 100 ng/ml of flagellin. Samples were collected at 0, 2, 6 and 24 hours and analyzed using NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection System 2.0. Control Data of NF-κB activity are reported as the fold induction of SEAP activity over untreated controls. Different letters mean significant difference (p<0.05).