Figure 5. JEV infection up-regulates the IFN signaling pathway in autophagy-deficient A549 cells.

(A) The cells were first transfected with the indicated siRNA, and 48 hours later the cells were then transfected with IFN β-Luc Firefly luciferase and an internal control, pRL-TK (Renilla luciferase). After 24 hours, the cells were infected with JEV at an MOI of 10. Finally, the cells were harvested at the indicated time, and dual luciferase activity was determined. (B) The cells were first transfected with the indicated siRNA, and 72 hours later the cells were infected at an MOI of 10. At different time points, total RNA was extracted for analysis. (C) The experiment was processed in parallel with (B), except that proteins used for immunoblotting were harvested at 24 hours post-infection. Three replicates were performed, and the results are presented as the means with SEM. The statistical significance of variations compared with the respective controls was calculated by t-test. *: P<0.05, **: P<0.01.