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. 2013 Jan 8;8(1):e52434. doi: 10.1371/journal.pone.0052434

Figure 2. Delaying the onset of reverse transcription does not increase viral sensitivity to hTRIM5α.

Figure 2

Untransduced U373-X4 cells and U373-X4 cells transduced with lentiviral vectors resulting in the overexpression of β-galactosidase (LacZ) or human TRIM5γ (TRIM5γ) were cultured overnight in the presence of 100 U/ml IFNα, and infected with 3 ng p24/well of the indicated recombinant VSV-pseudotyped viruses, which express Renilla luciferase in the place of Nef, and luciferase activity was measured 40 h after infection. Parallel cultures were maintained in the presence of 250 ng/ml NVP for the indicated times prior to washing the cells to remove NVP. Cultures not receiving NVP were washed 1 h after infection. In the left panels, results for cells not treated with NVP are expressed relative to RLU measured in untransduced U373-X4 cells. In the right panels, results for each cell line are expressed relative to RLU measured in cultures not treated with NVP. Shown are the mean ± SEM for three independent experiments performed using fresh viral stocks. ** indicates p<0.01 compared to U373-X4-LacZ cells.