Figure 5. γH2AX immunostaining in pronuclei of oocytes injected with up to four nuclei from freeze-dried lymphocytes.

NT = nuclear transfer, 4NT = 4 pronuclei (one fragmentated), PI  = pronuclei stained with propidium iodide; γH2AX = phosphorylated H2AX, a marker of DSBs and, indirectly, of DNA repair; Merge = PI+γH2AX. Scale bar = 20 µm.