Table 1. In vitro development of enucleated oocytes injected with nuclei from freeze-dried and fresh cells.
Donor cells | Cultured | Cleavage | Morula/Cleavage | Blastocyst/Cleavage |
Fresh lymphocytes | 356 | 124 (34.8%)a | 32 (25.8%)b | 25 (20.2%)c |
Freeze-dried lymphocytes | 825 | 218 (26.4%)a | 26 (11.9%)b | 19 (8.7%)c |
Embryos were cultured for 7 days. Reconstructed embryos were checked at day 1, 5, 6 and 7 for development;
p = 0.0035;
p = 0.001;
p = 0.0024 (χ2 test).