Table 3. Primers and Probes for Real Time PCR analysis of EBV gene expression.
EBV Gene | Primer Sequence (5′-3′) | *Probe Sequence (5′-3′) |
EBNA1 | GAT TCT GCA GCC CAG AGA GTA GTC | TCG TCG CAT CAT AGA CCG CCA GTA GAC |
TCG TCA GAC ATG ATT CAC ACT TAA AG | ||
EBNA2 | TAA CCA CCC AGC GCC AAT C | CAC CAC GTC ACA CGC CAG TGC TGG GT |
GTA GGC ATG ATG GCG GCA G | ||
EBNA3A | GAT TCT GCA GCC CAG AGA GTA GTC | CCC GGC CTG TCC TTG TCC ATT TTG |
CTT CTT CCA TGT TGT CAT CCA GG | ||
EBNA3B | GAT TCT GCA GCC CAG AGA GTA GTC | TAG ACC GCC AGT AGA CCT GGG AGC AGA |
CCA CGC TTT CTT CAT TAT TCA GGT | ||
EBNA3C | GAT TCT GCA GCC CAG AGA GTA GTC | AAG ACC CAC CAT GGA ATC ATT TGA AGG A |
CCA GGG TCC TGA TCA TGC TC | ||
LMP1 | TCA TCG CTC TCT GGA ATT TG | AGC ACA ATT CCA AGG AAC AAT GCC TGT C |
TCC AGA TAC CTA AGA CAA GTA AGC AC | ||
LMP2A | CTA CTC TCC ACG GGA TGA CTC AT | TGT TGC GCC CTA CCT CTT TTG GCT GGC G |
GGC GGT CAC AAC GGT ACT AAC T | ||
LMP2B | CGG GAG GCC GTG CTT TAG | TGT TGC GCC CTA CCT CTT TTG GCT GGC G |
GGC GGT CAC AAC GGT ACT AAC T | ||
BZLF1 | TTC CAC AGC CTG CAC CAG T | CAA CAG CCA GAA TCG CTG GAG GAA TGC G |
AGC AGC CAC CTC ACG GTA GT |
All probes were conjugated with 5′ FAM and a 3′ TAMRA quencher.