| Primary antibody |
Immunogen |
Manufacturer, catalog No., species |
Concentration/Dilution |
| CB1 |
Mouse CB1, C-terminal 31 aa (443–473, NM007726) |
Frontier Institute, CB1-Go-Af450, goat polyclonal (Fukudome et al., 2004) |
2 µg/ml |
| CB1 |
Mouse CB1, C-terminal 31 aa (443–473, NM007726) |
Frontier Institute, CB1-Rb-Af380, rabbit polyclonal (Uchigashima et al., 2007), |
2 µg/ml for immunohistochemistry, 0.5 µg/ml for western blot |
| MAP2 |
Rat brain microtubule associated proteins (MAPs) |
SIGMA, M4403, mouse monoclonal |
1∶500 |
| Synaptophysin |
Vesicular fraction of bovine brain |
Millipore, MAB5258, mouse monoclonal |
2 µg/ml |
| VGAT |
Mouse VGAT, 31–112 aa (BC052020) |
Frontier Institute, VGAT-Rb-Af500, rabbit polyclonal (Fukudome et al., 2004), |
2 µg/ml |
| VGluT1 |
Mouse VGluT1, C-terminal 531–560 aa (NM20309) |
Frontier Institute, VGluT1-Rb-Af500, rabbit polyclonal |
2 µg/ml |
| VGluT2 |
Mouse VGluT2, C-terminal 550–582 aa (BC038375) |
Frontier Institute, VGluT2-Rb-Af720, rabbit polyclonal |
2 µg/ml |
| GAPDH |
Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle |
Millipore, MAB374, mouse monoclonal |
0.05 µg/ml |
