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. 2013 Jan 8;8(1):e53328. doi: 10.1371/journal.pone.0053328

Figure 5. Functional analyses of the impact of Cul3 silencing on: A) proliferation, B) migration, and C) invasion.

Figure 5

The average of duplicate experiments of each functional assay with siRNAs against Cul3 versus the control siRNA is represented in each panel. D) Immunoblotting of proteins found differentially expressed by SILAC upon Cul3 silencing on T24T cells. The results suggested that the differential expression of several of these proteins would be likely regulated by Cul3. Antibodies displaying a single predominant band at the expected molecular weights were accepted: Cul3, Cullin 3; CAV1, Caveolin 1; FLNA, Filamin A; MSN, Moesin; EZR, Ezrin; NPM, Nucleophosmin; NUP133, Nucleoporin 133; IPO9. Importin 9; EGFR, Epidermal Growth Factor Receptor; GSN, Gelsolin; and α-tubulin, was used as the loading control.