Table 2. mRNA expression from laser captured neurons in the CA1 of the hippocampus, superior frontal gyrus, and visual cortex using Affymetrix Human Genome U133 plus 2.0 microarrays.
| Brain Region | Gene Name | Description | p-value | Fold Change | Mean ND | Mean AD |
| RAN | RAN, member RAS oncogene family | |||||
| Hippocampus | 5.82E−05 | −4.60E+00 | 1.01E+03 | 2.20E+02 | ||
| Superior Frontal Gyrus | 1.32E−02 | −5.01E+00 | 1.96E+03 | 3.91E+02 | ||
| Visual Cortex | 1.97E−02 | −2.08E+00 | 8.43E+02 | 4.05E+02 | ||
| RANBP1 | RAN binding protein 1 | |||||
| Hippocampus | 2.35E−03 | −2.47E+00 | 3.02E+02 | 1.22E+02 | ||
| Superior Frontal Gyrus | 2.74E−02 | −2.57E+00 | 3.83E+02 | 1.49E+02 | ||
| Visual Cortex | 3.85E−01 | −1.28E+00 | 1.90E+02 | 1.48E+02 | ||
| RANBP2 | RAN binding protein 2 | |||||
| Hippocampus | 7.14E−03 | −1.74E+00 | 7.96E+02 | 4.57E+02 | ||
| Superior Frontal Gyrus | 6.12E−02 | −1.91E+00 | 5.83E+02 | 3.06E+02 | ||
| Visual Cortex | 4.15E−02 | −1.22E+00 | 3.73E+02 | 3.06E+02 | ||
| RANBP5 | RAN binding protein 5 | |||||
| Hippocampus | 4.21E-02 | −1.49E+00 | 1.69E+02 | 1.14E+02 | ||
| Superior Frontal Gyrus | 2.26E−02 | −2.86E+00 | 2.65E+02 | 9.26E+01 | ||
| Visual Cortex | 8.82E−04 | −1.79E+00 | 1.62E+02 | 9.07E+01 | ||
| RANBP6 | RAN binding protein 6 | |||||
| Hippocampus | 8.21E+00 | 1.03E+00 | 1.15E+03 | 1.19E+03 | ||
| Superior Frontal Gyrus | 2.05E−02 | −3.02E+00 | 3.05E+03 | 1.01E+03 | ||
| Visual Cortex | 3.94E−02 | −1.47E+00 | 1.38E+03 | 9.39E+02 | ||
| RANBP9 | RAN binding protein 9 | |||||
| Hippocampus | 6.26E−05 | −3.06E+00 | 1.89E+02 | 6.18E+01 | ||
| Superior Frontal Gyrus | 1.57E−02 | −2.62E+00 | 1.98E+02 | 7.56E+01 | ||
| Visual Cortex | 1.71E−01 | −1.45E+00 | 1.05E+02 | 7.29E+01 | ||
| RANBP10 | RAN binding protein 10 | |||||
| Hippocampus | 1.74E−02 | −2.07E+00 | 1.42E+02 | 6.86E+01 | ||
| Superior Frontal Gyrus | 1.64E+00 | −1.36E+00 | 1.11E+02 | 8.14E+01 | ||
| Visual Cortex | 1.14E+00 | −1.19E+00 | 8.79E+01 | 7.37E+01 |
mRNA data from all three brain regions show a significant decreases in RAN and RAN binding proteins, with lesser amounts in the visual cortex, an area with only modest AD pathology [16]. With the exception of RANBP6 in the hippocampus, all other RAN binding proteins were significantly down in AD compared to controls.