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. 2013 Jan 8;8(1):e53264. doi: 10.1371/journal.pone.0053264

Figure 1. Selection of monoclonal antibodies.

Figure 1

(A) Amplified genes of antibody variable regions. The arrows indicate the target DNA bands. (B) Enrichment of rNcSAG1-specific Fab-phage. rNcSAG1 (0.5 µg/ml), and BSA (10 µg/ml) were immobilized on a 96-well microplate, respectively. ECL™ Anti-mouse IgG, Horseradish Peroxidase linked whole antibody (from sheep) was used as the secondary antibody. R0 stands for the original phage library, whereas R1, R2, and R3 stand for the amplified Fab-phage in rounds 1, 2, and 3 of biopanning, respectively. Experimental data were presented as average values with standard error (n = 3). (C) Screening of monoclonal Fab-phage. (D) Binding of Fab-phages to BSA and rNcSAG1.